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Article

Effects of mutations in the L-tryptophan binding pocket of the Trp RNA-binding attenuation protein of Bacillus subtilis.

The Journal of biological chemistry - 11 Feb 2000

Yakhnin A V, Trimble J J, Chiaro C R, Babitzke P

Abstract excerpt

The Bacillus subtilis tryptophan biosynthetic genes are regulated by the trp RNA-binding attenuation protein (TRAP). Cooperative binding of L-tryptophan activates TRAP so that it can bind to RNA. The crystal structure revealed that L-tryptophan forms nine hydrogen bonds with various amino acid residues of TRAP. We performed site-directed mutagenesis to determine the importance of several of these hydrogen bonds...

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Source

Identifiers

PMID: 10660627
DOI: 10.1074/jbc.275.6.4519

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Effects of mutations in the L-tryptophan binding pocket of the Trp RNA-binding attenuation protein of Bacillus subtilis.PMID 10660627Read ↗

The anti-trp RNA-binding attenuation protein (Anti-TRAP), AT, recognizes the tryptophan-activated RNA binding domain of the TRAP regulatory protein.Semantic proximity Read ↗

A temperature-sensitive trpS mutation interferes with trp RNA-binding attenuation protein (TRAP) regulation of trp gene expression in Bacillus subtilis.Semantic proximity Read ↗

The Bacillus subtilis TRAP protein can induce transcription termination in the leader region of the tryptophan biosynthetic (trp) operon independent of the trp attenuator RNA.Semantic proximity Read ↗

trp RNA-binding attenuation protein (TRAP)-trp leader RNA interactions mediate translational as well as transcriptional regulation of the Bacillus subtilis trp operon.Semantic proximity Read ↗

RNA structure inhibits the TRAP (trp RNA-binding attenuation protein)-RNA interaction.Semantic proximity Read ↗

The structure of trp RNA-binding attenuation protein.Semantic proximity Read ↗

Effects of tryptophan starvation on levels of the trp RNA-binding attenuation protein (TRAP) and anti-TRAP regulatory protein and their influence on trp operon expression in Bacillus subtilis.Semantic proximity Read ↗

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Identification of a Residue (Glu60) in TRAP Required for Inducing Efficient Transcription Termination at the trp Attenuator Independent of Binding Tryptophan and RNA.Semantic proximity Read ↗

Effect of amino acid alterations in the tryptophan-binding site of the trp repressor.Semantic proximity Read ↗

Creating hetero-11-mers composed of wild-type and mutant subunits to study RNA binding to TRAP.Semantic proximity Read ↗

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