Article
Selection of viral RNA-derived tRNA-like structures with improved valylation activities.
Biochemistry - 23 May 2000
Wientges J, Pütz J, Giegé R, Florentz C, Schwienhorst A
Abstract excerpt
The tRNA-like structure (TLS) of turnip yellow mosaic virus (TYMV) RNA was previously shown to be efficiently charged by yeast valyl-tRNA synthetase (ValRS). This RNA has a noncanonical structure at its 3'-terminus but mimics a tRNA L-shaped fold, including an anticodon loop containing the major identity nucleotides for valylation, and a pseudoknotted amino acid accepting domain. Here we describe an in vitro...
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