Article
Pseudouridylation of U(35) in the anticodon of Arabidopsis thaliana pre-tRNA(Tyr) depends on length rather than structure of an intron.
Biochimica et biophysica acta - 19 Mar 2002
Pieńkowska Joanna, Michałowski Daniel, Krzyzosiak Wlodzimierz J, Szweykowska-Kulińska Zofia
Abstract excerpt
In order to establish the structure and sequence requirements for pseudouridine (Psi(35)) biosynthesis in Arabidopsis thaliana tRNA(Tyr) five mutants of nuclear pre-tRNA(Tyr) have been prepared and analyzed: DeltaI-tRNA(Tyr) transcript depleted of an intron, and 5UI, 7UI, 9UI and 12UI transcripts containing tracts of five, seven, nine and 12 U residues, respectively, instead of the wild type tRNA(Tyr) intron. The...
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