Article
Determinants within an 18-amino-acid U1A autoregulatory domain that uncouple cooperative RNA binding, inhibition of polyadenylation, and homodimerization.
Molecular and cellular biology - 1 May 2003
Guan Fei, Palacios Daphne, Hussein Reem I, Gunderson Samuel I
Abstract excerpt
The human U1 snRNP-specific U1A protein autoregulates its own production by binding to and inhibiting the polyadenylation of its own pre-mRNA. Previous work demonstrated that a short sequence of U1A protein is essential for autoregulation and contains three distinct activities, which are (i) cooperative binding of two U1A proteins to a 50-nucleotide region of U1A pre-mRNA called polyadenylation-inhibitory element...
Topics
- Amino Acid Sequence
- Amino Acids
- Binding Sites
- DNA Mutational Analysis
- Dimerization
- HeLa Cells
- Homeostasis
- Humans
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Polyadenylation
- Polynucleotide Adenylyltransferase