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Article

Optimizing the heterologous expression of glutathione transferase.

Methods in enzymology - 1 Jan 2005

Mannervik Bengt

Abstract excerpt

The heterologous expression of a protein may be enhanced by silent mutations in the coding region of its corresponding DNA. This simple approach has been successfully used for optimized production of a number of glutathione-linked enzymes. For example, the yield of human glutathione transferase M2-2 was elevated by 140-fold in a clone isolated by immunoscreening of a library of plasmids with randomized synonymous...

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PMID: 16399391
DOI: 10.1016/S0076-6879(05)01016-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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