Article
Use of silent mutations in cDNA encoding human glutathione transferase M2-2 for optimized expression in Escherichia coli.
Protein expression and purification - 1 Oct 1999
Johansson A S, Bolton-Grob R, Mannervik B
Abstract excerpt
Heterologous expression of human glutathione transferase M2-2 (GST M2-2) using Escherichia coli was improved 140-fold by mutating the cDNA expressing the enzyme. Expression of GST M2-2 from this cDNA clone, pKHXhGM2, generated approximately 190 mg protein per liter of bacterial culture, corresponding to approximately 12% of the total amount of soluble protein. The high-level-expressing cDNA was generated by...
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