Glutathione transferases with novel active sites isolated by phage display from a library of random mutants.

doi:10.1006/jmbi.1995.0362

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Article

Glutathione transferases with novel active sites isolated by phage display from a library of random mutants.

Journal of molecular biology - 7 Jul 1995

Widersten M, Mannervik B

Abstract excerpt

Human glutathione transferase A1-1 can be expressed as a fusion protein with coat protein III of filamentous phage f1 in a form that allows selection among variant mutant forms based on specific adsorption to immobilized active-site ligands. A library of mutant enzymes differing in the active-sit...

Topics

Amino Acid Sequence
Base Sequence
Binding Sites
DNA Mutational Analysis
Glutathione Transferase
Humans
Inovirus
Isoenzymes
Ligands
Molecular Sequence Data
Mutation
Peptides
Recombinant Fusion Proteins
Substrate Specificity

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