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Article

Glutathione transferases with novel active sites isolated by phage display from a library of random mutants.

Journal of molecular biology - 7 Jul 1995

Widersten M, Mannervik B

Abstract excerpt

Human glutathione transferase A1-1 can be expressed as a fusion protein with coat protein III of filamentous phage f1 in a form that allows selection among variant mutant forms based on specific adsorption to immobilized active-site ligands. A library of mutant enzymes differing in the active-site region was generated by random mutagenesis of ten amino acid residues involved in the binding of electrophilic...

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Identifiers

PMID: 7608963
DOI: 10.1006/jmbi.1995.0362

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Glutathione transferases with novel active sites isolated by phage display from a library of random mutants.PMID 7608963Read ↗

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