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Article

Evolution of differential substrate specificities in Mu class glutathione transferases probed by DNA shuffling.

Journal of molecular biology - 26 Mar 1999

Hansson L O, Bolton-Grob R, Massoud T, Mannervik B

Abstract excerpt

A library of variant enzymes was created by combined shuffling of the DNA encoding the human Mu class glutathione transferases GST M1-1 and GST M2-2. The parental GSTs are 84 % sequence identical at the protein level, but their specific activities with the substrates aminochrome and 2-cyano-1,3-dimethyl-1-nitrosoguanidine (cyanoDMNG) differ by more than 100-fold. Aminochrome is of particular interest as an...

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PMID: 10080890
DOI: 10.1006/jmbi.1999.2607

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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