Article
Optimized heterologous expression of the polymorphic human glutathione transferase M1-1 based on silent mutations in the corresponding cDNA.
Protein expression and purification - 1 Jun 1996
Widersten M, Huang M, Mannervik B
Abstract excerpt
An expression clone for large-scale production of the polymorphic human glutathione transferase (GST) M1-1 has been developed. Heterologous expression in Escherichia coli afforded a yield of 100 mg of GST M1-1 per 3 liters of culture medium, corresponding to an approximately 10-fold increased yie...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Chromatography, Agarose
- DNA Primers
- DNA, Complementary
- DNA, Recombinant
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression Regulation, Enzymologic
- Gene Library
- Glutathione Transferase
- Humans
- Molecular Sequence Data
- Mutation
- Plasmids
- Polymerase Chain Reaction
- Polymorphism, Genetic