Article
Evaluation of dried blood spot specimens for HIV-1 drug-resistance testing using the Trugene HIV-1 genotyping assay.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 Apr 2008
Hallack Renee, Doherty Lauren E, Wethers Judith A, Parker Monica M
Abstract excerpt
BACKGROUND: Efforts to simplify the collection and shipping of specimens for HIV drug-resistance testing in resource-limited settings are needed as antiretroviral therapy increases worldwide. OBJECTIVE: To evaluate the reliability and practicality of using dried blood spots (DBS) for HIV-1 drug-resistance testing with the Trugene HIV-1 genotyping assay. STUDY DESIGN: Nucleic acids from 33 DBS and counterpart...
Topics
- Amino Acid Substitution
- Blood
- Drug Resistance, Viral
- Genotype
- HIV-1
- Humans
- Microbial Sensitivity Tests
- Mutation, Missense
- Plasma
- Reproducibility of Results
- Sensitivity and Specificity
- Specimen Handling