Article
On the functional role of the Tyr-639 residue of bacteriophage T7 RNA polymerase.
FEBS letters - 20 Jul 1992
Rechinsky V O, Kostyuk D A, Tunitskaya V L, Kochetkov S N
Abstract excerpt
Substitution of Asp for a Tyr residue normally present at position 639 of the bacteriophage T7 RNA polymerase leads to a drastic drop in the enzymatic activity. This mutation does not affect the enzyme-promoter interaction but decreases the ability of the RNA polymerase to discriminate between GTP and ATP molecules, resulting in a decrease in the rate of the incorporation of the nucleotide into the RNA chain.
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