Article
On the functional role of the Tyr-639 residue of bacteriophage T7 RNA polymerase.
FEBS letters - 20 Jul 1992
Rechinsky V O, Kostyuk D A, Tunitskaya V L, Kochetkov S N
Abstract excerpt
Substitution of Asp for a Tyr residue normally present at position 639 of the bacteriophage T7 RNA polymerase leads to a drastic drop in the enzymatic activity. This mutation does not affect the enzyme-promoter interaction but decreases the ability of the RNA polymerase to discriminate between GT...
Topics
- Amino Acid Sequence
- Binding, Competitive
- DNA-Directed RNA Polymerases
- Kinetics
- Molecular Sequence Data
- Mutation
- T-Phages
- Tyrosine
- Viral Proteins