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Article

A critical residue selectively recruits nucleotides for t7 RNA polymerase transcription fidelity control.

Biophysical journal - 4 Nov 2014

Duan Baogen, Wu Shaogui, Da Lin-Tai, Yu Jin

Abstract excerpt

Nucleotide selection is essential for fidelity control in gene replication and transcription. Recent work on T7 RNA polymerase suggested that a small posttranslocation free energy bias stabilizes Tyr(639) in the active site to aid nucleotide selection. However, it was not clear exactly how Tyr(639) assists the selection. Here we report a molecular-dynamics simulation study revealing atomistic detail of this...

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Identifiers

PMID: 25418098
DOI: 10.1016/j.bpj.2014.09.038
PMCID: PMC4223216

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Source receipt: pubmed, retrieved 7 Sept 2026.

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