Article
Effects of mutations of the bulged nucleotide in the conserved P7 pairing element of the phage T4 td intron on ribozyme function.
Biochemistry - 2 Apr 1991
Schroeder R, von Ahsen U, Belfort M
Abstract excerpt
The P7 element of group I introns contains a semiconserved "bulged" nucleotide, a C in group IA introns (nt 870 in the td intron) and an A in group IB introns [Cech, T.R. (1988) Gene 73, 259-271]. Variants U870, G870, and A870, isolated by a combination of in vitro and in vivo genetic strategies,...
Topics
- Base Sequence
- Chromosome Deletion
- Escherichia coli
- Genetic Variation
- Guanosine Triphosphate
- Introns
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Nucleic Acid Conformation
- Oligonucleotide Probes
- Plasmids
- RNA Precursors
- RNA Splicing
- RNA, Catalytic
- T-Phages
- Transcription, Genetic