Article
DNA light-strand preferential recognition of human mitochondria transcription termination factor mTERF.
Journal of biochemistry and molecular biology - 30 Nov 2005
Nam Sang-Chul, Kang Changwon
Abstract excerpt
Transcription termination of the human mitochondrial genome requires specific binding to termination factor mTERF. In this study, mTERF was produced in E. coli and purified by two-step chromatography. mTERF-binding DNA sequences were isolated from a pool of randomized sequences by the repeated selection of bound sequences by gel-mobility shift assay and polymerase chain reaction. Sequencing and comparison of the...
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