Article
Androgen receptor gene mutations in human prostate cancer.
Proceedings of the National Academy of Sciences of the United States of America - 15 Jul 1992
Newmark J R, Hardy D O, Tonb D C, Carter B S, Epstein J I, Isaacs W B, Brown T R, Barrack E R
Abstract excerpt
We screened human prostate cancer tissues for the presence of somatic mutations in the hormone binding domain of the androgen receptor (AR) gene. Exons E-H were amplified from genomic DNA using the polymerase chain reaction and analyzed by denaturing gradient gel electrophoresis (DGGE), which separates DNA fragments that differ by only a single base. We detected a mutation in exon E of the hormone binding domain...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Humans
- In Vitro Techniques
- Male
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction
- Prostatic Neoplasms