Article
Isolation and characterization of PEP5, a gene essential for vacuolar biogenesis in Saccharomyces cerevisiae.
Genetics - 1 Aug 1990
Woolford C A, Dixon C K, Manolson M F, Wright R, Jones E W
Abstract excerpt
pep5 mutants of Saccharomyces cerevisiae accumulate inactive precursors to the vacuolar hydrolases. The PEP5 gene was isolated from a genomic DNA library by complementation of the pep5-8 mutation. Deletion analysis localized the complementing activity to a 3.3-kb DNA fragment. DNA sequence analys...
Topics
- Alkaline Phosphatase
- Amino Acid Sequence
- Base Sequence
- Codon
- Fungal Proteins
- Genes, Fungal
- Genetic Complementation Test
- Hydrolases
- Immunoblotting
- Microscopy, Electron
- Molecular Sequence Data
- Mutation
- Plasmids
- Restriction Mapping
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Transcription, Genetic
- Vacuoles