Article
High-level expression and purification of recombinant SCF ubiquitin ligases.
Methods in enzymology - 1 Jan 2005
Li Ti, Pavletich Nikola P, Schulman Brenda A, Zheng Ning
Abstract excerpt
The SCF complexes are the prototype of a superfamily of cullin-dependent ubiquitin ligases, which regulate diverse cellular functions by promoting the ubiquitination of a large number of regulatory and signaling proteins. The SCF complexes are organized by the elongated scaffold protein subunit Cul1, which interacts with the Rbx1 RING finger protein at one end and the Skp1 adaptor protein at the other. By binding...
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