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4G cloning: rapid gene assembly for expression of multisubunit protein complexes in diverse hosts

2024-06-17

Abstract excerpt

Multi-subunit protein complexes are at the heart of many cellular processes, and studying their biochemical activities and structures in vitro requires their reconstitution by recombinant expression and purification. Obtaining targets at sufficient purity and scale typically requires the screening of several protein variants and expression hosts. Existing cloning strategies allow to produce constructs for co-expr...

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Literature Corpus work
6e9598c6-383c-54e9-ba60-fdbd2aaacb41
DOI
10.1101/2024.06.17.599261
Open publication

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4G cloning: rapid gene assembly for expression of multisubunit protein complexes in diverse hostsDOI 10.1101/2024.06.17.599261
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