Article
Binding of the Bacillus subtilis LexA protein to the SOS operator.
Nucleic acids research - 1 Jan 2005
Groban Eli S, Johnson Martha B, Banky Poopak, Burnett Peta-Gaye G, Calderon Georgina L, Dwyer Erica C, Fuller Shakierah N, Gebre Biniam, King Leah M, Sheren Ila N, Von Mutius Lindi D, O'Gara Thomas M, Lovett Charles M
Abstract excerpt
The Bacillus subtilis LexA protein represses the SOS response to DNA damage by binding as a dimer to the consensus operator sequence 5'-CGAACN(4)GTTCG-3'. To characterize the requirements for LexA binding to SOS operators, we determined the operator bases needed for site-specific binding as well as the LexA amino acids required for operator recognition. Using mobility shift assays to determine equilibrium...
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