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Article

Efficient transformation of Lactococcus lactis IL1403 and generation of knock-out mutants by homologous recombination.

Journal of basic microbiology - 1 Jun 2007

Gerber Simon D, Solioz Marc

Abstract excerpt

Lactococcus lactis IL1403 is a Gram-positive bacterium of great biotechnological interest for food grade applications. Its use is however hampered by the difficulty to efficiently transform this strain. We here describe a detailed, optimized electrotransformation protocol which yields a transformation efficiency of 10(6) cfu/microg of DNA with the two E. coli Gram-positive shuttle vectors pC3 and pVA838. The...

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PMID: 17518422
DOI: 10.1002/jobm.200610297

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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