Article
An actin-binding site containing a conserved motif of charged amino acid residues is essential for the morphogenic effect of villin.
Cell - 10 Jul 1992
Friederich E, Vancompernolle K, Huet C, Goethals M, Finidori J, Vandekerckhove J, Louvard D
Abstract excerpt
The actin-binding protein villin induces microvillus growth and reorganization of the cytoskeleton in cells that do not normally produce this protein. Transfection of mutagenized villin cDNAs into CV-1 cells was used to show that a conserved, COOH-terminally located cluster of charged amino acid residues (KKEK) is crucial for the morphogenic activity of villin in vivo. In vitro experiments with a 22 amino acid...
Topics
- Actins
- Amino Acid Sequence
- Binding Sites
- Carrier Proteins
- Cytoskeleton
- Microfilament Proteins
- Molecular Sequence Data
- Morphogenesis
- Mutation
- Peptide Fragments
- Transfection