An actin footprint on villin. Single site substitutions in a cluster of basic residues inhibit the actin severing but not capping activity of villin.

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Article

An actin footprint on villin. Single site substitutions in a cluster of basic residues inhibit the actin severing but not capping activity of villin.

The Journal of biological chemistry - 25 Jun 1992

de Arruda M V, Bazari H, Wallek M, Matsudaira P

Abstract excerpt

Villin is a multidomain protein that severs, caps, and bundles actin filaments. We employed a chemical modification/cleavage strategy to identify residues whose chemical reactivities are reduced when villin is complexed with actin. We found that actin protects 3 methionine residues, Met125, Met379, and Met711 from oxidation by N-chlorosuccinimide. Because Met125 lies within the actin-severing domain of villin...

Topics

Actins
Amino Acid Sequence
Animals
Carrier Proteins
Chickens
Circular Dichroism
Cyanogen Bromide
Electrochemistry
Hydrogen-Ion Concentration
Immunoblotting
Methionine

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Microfilament Proteins

Microscopy, Electron

Models, Molecular

Molecular Sequence Data

Mutation

Oxidation-Reduction

Succinimides

Source

Open in PubMed

Identifiers

PMID: 1618806

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1618806-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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