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Article

Construction of an effective protein expression system using the tpl promoter in Escherichia coli.

Biotechnology letters - 1 Sept 2005

Koyanagi Takashi, Katayama Takane, Hirao Ai, Suzuki Hideyuki, Kumagai Hidehiko

Abstract excerpt

An effective protein expression system was constructed in Escherichia coli using the promoter of the tyrosine phenol-lyase (tpl) gene of Erwinia herbicola. This system involves a mutant form of the TyrR protein with an enhanced ability to activate tpl and the TutB protein with an ability to transport L-tyrosine (an inducer of Tpl). The highest expression level obtained for this system was more than twice that...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 16215823
DOI: 10.1007/s10529-005-0216-4

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SDS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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