Article
Hyperproduction of 3,4-dihydroxyphenyl-L-alanine (L-Dopa) using Erwinia herbicola cells carrying a mutant transcriptional regulator TyrR.
Bioscience, biotechnology, and biochemistry - 1 May 2009
Koyanagi Takashi, Katayama Takane, Suzuki Hideyuki, Onishi Akiko, Yokozeki Kenzo, Kumagai Hidehiko
Abstract excerpt
In the last few decades, enzymatic production of 3,4-dihydroxyphenyl-L-alanine (L-dopa) using tyrosine phenol-lyase (Tpl) has been industrialized. This method has an intrinsic problem of tyrosine contamination because Tpl is synthesized under tyrosine-induced conditions. Herein, we constructed a hyper-L-dopa-producing strain by exploiting a mutant TyrR, an activator of tpl. The highest productivity was obtained...
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