Article
VTR expression cassettes for engineering conditional phenotypes in Pseudomonas: activity of the Pu promoter of the TOL plasmid under limiting concentrations of the XylR activator protein.
Gene - 12 Jun 1996
Pérez-Martín J, de Lorenzo V
Abstract excerpt
A simplified procedure to construct recombinant Pseudomonas putida (Pp) and related bacteria, which transcribe conditionally specific genes inserted into their chromosome in response to lac inducers such as IPTG, has been developed. The method is based on the so-called VTR expression cassettes. These are three small (1.98-kb) DNA segments engineered as NotI restriction fragments that include a lacIq gene along...
Topics
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- DNA Primers
- DNA-Binding Proteins
- Molecular Sequence Data
- Phenotype
- Plasmids
- Promoter Regions, Genetic
- Pseudomonas putida
- Transcription Factors