Co-expression of yeast amber suppressor tRNATyr and tyrosyl-tRNA synthetase in Escherichia coli: possibility to expand the genetic code.

doi:10.1093/oxfordjournals.jbchem.a022221

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Article

Co-expression of yeast amber suppressor tRNATyr and tyrosyl-tRNA synthetase in Escherichia coli: possibility to expand the genetic code.

Journal of biochemistry - 1 Dec 1998

Ohno S, Yokogawa T, Fujii I, Asahara H, Inokuchi H, Nishikawa K

Abstract excerpt

An efficient system was developed for the co-expression of a yeast tRNATyr/tyrosyl-tRNA synthetase (TyrRS) pair in Escherichia coli. Analysis of suppression patterns using several sets of E. coli and lambda phage mutants indicated that the expressed yeast suppressor tRNATyr was aminoacylated only...

Topics

Bacteriophage lambda
Escherichia coli
Genes, Suppressor
Genetic Engineering
Mutation
RNA, Transfer, Tyr
Transformation, Bacterial
Tyrosine-tRNA Ligase
Yeasts
beta-Galactosidase

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