Article
Screening through the PLICable promoter toolbox enhances protein production in Escherichia coli.
Biotechnology journal - 1 Dec 2016
Yang Jianhua, Ruff Anna Joëlle, Hamer Stefanie Nicole, Cheng Feng, Schwaneberg Ulrich
Abstract excerpt
Escherichia coli is a common host for recombinant protein production in which production titers are highly dependent on the employed expression system. Promoters are thereby a key element to control gene expression levels. In this study, a novel PLICable promoter toolbox was developed which enables in a single cloning step and after a screening experiment to identify out of ten IPTG-inducible promoters (T7, A3,...
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