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Article

Analysis of coumarin 7-hydroxylation activity of cytochrome P450 2A6 using random mutagenesis.

The Journal of biological chemistry - 2 Dec 2005

Kim Donghak, Wu Zhong-Liu, Guengerich F Peter

Abstract excerpt

Cytochrome P450 (P450) 2A6 is an important human enzyme involved in the metabolism of many xenobiotic chemicals including coumarin, indole, nicotine, and carcinogenic nitrosamines. A combination of random mutagenesis and high-throughput screening was used in the analysis of P450 2A6, utilizing a fluorescent coumarin 7-hydroxylation assay. The steady-state kinetic parameters (k(cat) and Km) for coumarin...

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Source

Identifiers

PMID: 16207711
DOI: 10.1074/jbc.M508171200

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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