Article
Whole genome amplification of buccal cytobrush DNA collected for molecular epidemiology studies.
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals - 1 Jan 2000
Moore L E, Bergen A W, Haque K A, Qi Y, Castle P, Chanock S J, Egan K, Newcomb P, Titus-Ernstoff L, Alguacil J, Rothman N, Garcia-Closas M
Abstract excerpt
When cytobrush buccal cell samples have been collected as a genomic DNA (gDNA) source for an epidemiological study, whole genome amplification (WGA) can be critical to maintain sufficient DNA for genotyping. We evaluated REPLI-g WGA using gDNA from two paired cytobrushes (cytobush 'A' kept in a cell lysis buffer, and 'B' dried and kept at room temperature for 3 days, and frozen until DNA extraction) in a pilot...
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