Article
The role of firefly luciferase C-terminal domain in efficient coupling of adenylation and oxidative steps.
FEBS letters - 15 Aug 2005
Ayabe Keiichi, Zako Tamotsu, Ueda Hiroshi
Abstract excerpt
The N-terminal domain (N-domain) of the firefly luciferase from Photinus pyraris has weak luminescence activity, and shows a unique light emitting profile with very long rise time of more than several minutes. Through a sensitive assay of the reaction intermediate luciferyl-adenylate (LH2-AMP), we found that the slow increase in the N-domain luminescence faithfully reflected the concentration of dissociated...
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