Article
Bioluminescence spectra of native and mutant firefly luciferases as a function of pH.
Biochemistry. Biokhimiia - 1 Nov 2005
Ugarova N N, Maloshenok L G, Uporov I V, Koksharov M I
Abstract excerpt
Bioluminescence spectra of the wild-type recombinant Luciola mingrelica firefly luciferase and its mutant form with the His433Tyr point mutation were obtained within the pH 5.6-10.2 interval. The spectra are shown to be a superposition of the spectra of the three forms of the electronically excited reaction product oxyluciferin: ketone (lambdamax = 618 nm), enol (lambdamax = 587 nm), and enolate-ion (lambdamax =...
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