Article
Spectroscopic study of firefly oxyluciferin in an enzymatic environment on the basis of stability monitoring.
The journal of physical chemistry. B - 27 Feb 2014
Wang Yu, Hayamizu Yuhei, Akiyama Hidefumi
Abstract excerpt
To understand the influence of the enzyme microenvironment on the properties of the emitter oxyluciferin (OL) in firefly bioluminescence, we investigated the spectroscopic characteristics of OL in a complex with the enzyme luciferase formed in a consumed reaction mixture. By monitoring the in situ absorption spectra, we analyzed the enzymatic synthesis and the stability of OL in luciferase environment. The...
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