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Article

Sensitive sequencing method for KRAS mutation detection by Pyrosequencing.

The Journal of molecular diagnostics : JMD - 1 Aug 2005

Ogino Shuji, Kawasaki Takako, Brahmandam Mohan, Yan Liying, Cantor Mami, Namgyal Chungdak, Mino-Kenudson Mari, Lauwers Gregory Y, Loda Massimo, Fuchs Charles S

Abstract excerpt

Both benign and malignant tumors represent heterogenous tissue containing tumor cells and non-neoplastic mesenchymal and inflammatory cells. To detect a minority of mutant KRAS alleles among abundant wild-type alleles, we developed a sensitive DNA sequencing assay using Pyrosequencing, ie, nucleotide extension sequencing with an allele quantification capability. We designed our Pyrosequencing assay for use with...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 16049314
DOI: 10.1016/S1525-1578(10)60571-5
PMCID: PMC1867544

Genes

KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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