Article
Fundamentals of pyrosequencing.
Archives of pathology & laboratory medicine - 1 Sept 2013
Harrington Colleen T, Lin Elaine I, Olson Matthew T, Eshleman James R
Abstract excerpt
CONTEXT: DNA sequencing is critical to identifying many human genetic disorders caused by DNA mutations, including cancer. Pyrosequencing is less complex, involves fewer steps, and has a superior limit of detection compared with Sanger sequencing. The fundamental basis of pyrosequencing is that pyrophosphate is released when a deoxyribonucleotide triphosphate is added to the end of a nascent strand of DNA....
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