Article
Effects of edd and pgi disruptions on inosine accumulation in Escherichia coli.
Bioscience, biotechnology, and biochemistry - 1 Jul 2005
Shimaoka Megumi, Kawasaki Hisashi, Takenaka Yasuhiro, Kurahashi Osamu, Matsui Hiroshi
Abstract excerpt
Using an inosine-producing mutant of Escherichia coli, the contributions of the central carbon metabolism for overproducing inosine were investigated. Sodium gluconate instead of glucose was tested as a carbon source to increase the supply of ribose-5-phosphate through the oxidative pentose phosphate pathway. The edd (6-phosphogluconate dehydrase gene)-disrupted mutant accumulated 2.5 g/l of inosine from 48 g/l...
Topics
- Adenine
- Aminohydrolases
- Cell Division
- Escherichia coli
- Gluconates
- Glucose
- Glucose-6-Phosphate Isomerase
- Glucosephosphate Dehydrogenase
- Hydro-Lyases
- Inosine
- Mutation