Article
De novo engineering and metabolic flux analysis of inosine biosynthesis in Bacillus subtilis.
Biotechnology letters - 1 Aug 2011
Li Haojian, Zhang Guoqiang, Deng Aihua, Chen Ning, Wen Tingyi
Abstract excerpt
Wild-type B. subtilis strain W168 was de novo engineered for inosine biosynthesis. Inactivation of deoD and purA led to 0.15 ± 0.04 and 6.44 ± 0.39 g inosine/l yields, respectively. The deoD purA double mutant accumulated 7.6 ± 0.34 g inosine/l, with a 4.7% (w/w) conversion ratio from glucose to inosine. Comparative metabolic flux analysis revealed that the fluxes from inosine to hypoxanthine and from inosine...
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