Article
Novel electrophoretic protocol for collection of mutations in the lambda light chain immunoglobulin gene in a human B-lymphoblastoid cell strain.
Teratogenesis, carcinogenesis, and mutagenesis - 1 Jan 1995
McFarland R D, Smith G J
Abstract excerpt
Spontaneous and chemically induced mutation was examined in the lambda light chain immunoglobulin gene in a human B-lymphoblastoid cell strain (T5-1). The hemizygous lambda gene is a unique mutational target gene which codes for a protein that is both expressed on the cell membrane and secreted. Mutations in the lambda gene were detected by analysis of western blots of isoelectric focusing gel electrophoresis of...
Topics
- B-Lymphocytes
- Blotting, Western
- Electrophoresis, Agar Gel
- Flow Cytometry
- Genes, Immunoglobulin
- Humans
- Immunoglobulin Light Chains
- Immunophenotyping
- Isoelectric Focusing
- Methylnitronitrosoguanidine