Article
[Comparative study on detection of hepatitis B virus mutants in precore region with two methods].
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology - 1 Mar 2007
Liu Yue-hui, Ding Jing-juan
Abstract excerpt
OBJECTIVE: To establish a mismatched polymerase chain reaction restricted fragment length polymorphism (mPCR-RFLP) method for detection of hepatitis B virus (HBV) mutation in precore A1896, and compare with direct sequencing for evaluating its applicability. METHODS: According to the principle of mPCR, 194bp gene fragments in HBV precore region was amplified. The products of PCR were digested by Bsu36I and...
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