Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Genetic modification and variations in solvent increase the sensitivity of the yeast RAD54-GFP genotoxicity assay.

Mutagenesis - 1 Sept 2005

Walsh L, Hastwell P W, Keenan P O, Knight A W, Billinton N, Walmsley R M

Abstract excerpt

The yeast (Saccharomyces cerevisiae) RAD54-GFP DNA repair reporter assay (GreenScreen assay, GSA) can be used for early genotoxicity screening in drug discovery. During the initial validation of this preregulatory assay, a subset of known genotoxic compounds that did not give reproducibly clear positive GSA results was identified. Cell permeability, inherent drug resistance mechanisms, metabolic activation and...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Wall
Share to Cytochrome P-450 CYP3A
Share to DNA Helicases

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to DNA Repair Enzymes

Share to Ethanol

Share to Mutagenicity Tests

Share to Mutagens

Share to Solvents

Source

Identifiers

PMID: 15985442
DOI: 10.1093/mutage/gei044

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Genetic modification and variations in solvent increase the sensitivity of the yeast RAD54-GFP genotoxicity assay.PMID 15985442Read ↗

Genomic phenotyping of the essential and non-essential yeast genome detects novel pathways for alkylation resistance.Semantic proximity Read ↗

Mitochondrial perturbation reduces susceptibility to xenobiotics through altered efflux in Candida albicansSemantic proximity Read ↗

Genomic phenotyping by barcode sequencing broadly distinguishes between alkylating agents, oxidizing agents, and non-genotoxic agents, and reveals a role for aromatic amino acids in cellular recovery after quinone exposure.Semantic proximity Read ↗

Bacterial mutagenicity screening in the pharmaceutical industry.Semantic proximity Read ↗

Continued progress in developing the Pig-a gene mutation assay.Semantic proximity Read ↗

Phenotypic yeast growth analysis for chronic toxicity testing.Semantic proximity Read ↗

Assessing the impact of different solvents in the bacterial reverse mutation test.Semantic proximity Read ↗

The BlueScreen-384 assay as an indicator of genotoxic hazard potential in early-stage drug discovery.Semantic proximity Read ↗

Molecular analysis of hprt mutations induced by chromium picolinate in CHO AA8 cells.Semantic proximity Read ↗

Toxicity, mutagenicity and transport in Saccharomyces cerevisiae of three popular DNA intercalating fluorescent dyes.Semantic proximity Read ↗

Cytotoxic and genotoxic properties of tert.-butyl-p-quinone (TBQ) in an in vitro assay system with Chinese hamster V79 cells and in strain D7 of Saccharomyces cerevisiae.Semantic proximity Read ↗

Genetic manipulation of yeast to identify genes involved in regulation of chemosensitivity.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.