Genetic modification and variations in solvent increase the sensitivity of the yeast RAD54-GFP genotoxicity assay.

doi:10.1093/mutage/gei044

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Article

Genetic modification and variations in solvent increase the sensitivity of the yeast RAD54-GFP genotoxicity assay.

Mutagenesis - 1 Sept 2005

Walsh L, Hastwell P W, Keenan P O, Knight A W, Billinton N, Walmsley R M

Abstract excerpt

The yeast (Saccharomyces cerevisiae) RAD54-GFP DNA repair reporter assay (GreenScreen assay, GSA) can be used for early genotoxicity screening in drug discovery. During the initial validation of this preregulatory assay, a subset of known genotoxic compounds that did not give reproducibly clear positive GSA results was identified. Cell permeability, inherent drug resistance mechanisms, metabolic activation and...

Topics

ATP-Binding Cassette Transporters
Cell Wall
Cytochrome P-450 CYP3A
Cytochrome P-450 Enzyme System
DNA Helicases
DNA Repair
DNA Repair Enzymes
Ethanol
Gene Deletion
Genes, Reporter
Green Fluorescent Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Mutagenicity Tests

Mutagens

Mutation

Saccharomyces cerevisiae Proteins

Sensitivity and Specificity

Solvents

Source

Open in PubMed

Identifiers

PMID: 15985442
DOI: 10.1093/mutage/gei044

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