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Article

Asymmetric PCR increases efficiency of melting peak analysis on the LightCycler.

Clinical biochemistry - 1 Aug 2005

Szilvási Anikó, Andrikovics Hajnalka, Kalmár Lajos, Bors András, Tordai Attila

Abstract excerpt

OBJECTIVES: To systematically analyze the effects of asymmetric PCR on LightCycler melting analyses of four different allelic-discrimination systems and to reduce an inconsistent non-specific melting peak observed during factor V Leiden genotyping. DESIGN AND METHODS: PCR amplifications and melting analyses were carried out with various oligonucleotide concentrations and ratios. To monitor the efficiency,...

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Identifiers

PMID: 15982647
DOI: 10.1016/j.clinbiochem.2005.04.015

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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