Article
Multiplex Asymmetric PCR by Combining the Amplification Refractory Mutation System with the Homo-Tag-Assisted Nondimer System.
Analytical chemistry - 4 Jun 2024
Liu Ying, Liu Yinghua, Guo Liu, Wu Yazhe, Wang Yafang, Xu Lingzhen, Xu Mingzhu, Huang Siyu, Chen Ping, Wang Ting, Huang Qiuying, Li Qingge
Abstract excerpt
Asymmetric PCR is widely used to produce single-stranded amplicons (ss-amplicons) for various downstream applications. However, conventional asymmetric PCR schemes are susceptible to events that affect primer availability, which can be exacerbated by multiplex amplification. In this study, a new multiplex asymmetric PCR approach that combines the amplification refractory mutation system (ARMS) with the...
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