Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

MuPlex: multi-objective multiplex PCR assay design.

Nucleic acids research - 1 Jul 2005

Rachlin John, Ding Chunming, Cantor Charles, Kasif Simon

Abstract excerpt

We have developed a web-enabled system called MuPlex that aids researchers in the design of multiplex PCR assays. Multiplex PCR is a key technology for an endless list of applications, including detecting infectious microorganisms, whole-genome sequencing and closure, forensic analysis and for enabling flexible yet low-cost genotyping. However, the design of a multiplex PCR assays is computationally challenging...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Algorithms
Share to DNA Primers
Share to Internet

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Polymerase Chain Reaction

Share to Polymorphism, Single Nucleotide

Share to Sequence Analysis, DNA

Share to Software

Share to User-Computer Interface

Source

Open in PubMed Open full text

Identifiers

PMID: 15980531
DOI: 10.1093/nar/gki377
PMCID: PMC1160138

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

MuPlex: multi-objective multiplex PCR assay design.PMID 15980531Read ↗

Computational tradeoffs in multiplex PCR assay design for SNP genotyping.Semantic proximity Read ↗

Scaling Variant-Aware Multiplex Primer DesignSemantic proximity Read ↗

primerJinn: a tool for rationally designing multiplex PCR primer sets for amplicon sequencing and performing in silico PCRSemantic proximity Read ↗

CalcDalton: a tool for multiplex genotyping primer design for single-base extension reactions using cleavable primers.Semantic proximity Read ↗

MPD: multiplex primer design for next-generation targeted sequencing.Semantic proximity Read ↗

PrimerMapper: high throughput primer design and graphical assembly for PCR and SNP detection.Semantic proximity Read ↗

High-throughput PCR assay design for targeted resequencing using primerXL.Semantic proximity Read ↗

primerJinn – a tool for rationally designing multiplex PCR primer sets and in silico PCRSemantic proximity Read ↗

URAdime – a tool for analyzing primer sequences in sequencing data to identify dimers and super-ampliconsSemantic proximity Read ↗

NGS-PrimerPlex: High-throughput primer design for multiplex polymerase chain reactions.Semantic proximity Read ↗

Primer-design for multiplexed genotyping.Semantic proximity Read ↗

DFold: PCR design that minimizes secondary structure and optimizes downstream genotyping applications.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.