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NGS-PrimerPlex: High-throughput primer design for multiplex polymerase chain reactions.

PLoS computational biology - 1 Dec 2020

Kechin Andrey, Borobova Viktoria, Boyarskikh Ulyana, Khrapov Evgeniy, Subbotin Sergey, Filipenko Maxim

Abstract excerpt

Multiplex polymerase chain reaction (PCR) has multiple applications in molecular biology, including developing new targeted next-generation sequencing (NGS) panels. We present NGS-PrimerPlex, an efficient and versatile command-line application that designs primers for different refined types of amplicon-based genome target enrichment. It supports nested and anchored multiplex PCR, redistribution among multiplex...

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PMID: 33378360
DOI: 10.1371/journal.pcbi.1008468
PMCID: PMC7802936

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