Genetic manipulations restored the growth fitness of reduced-genome Escherichia coli.

doi:10.1016/j.jbiosc.2013.01.010

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Genetic manipulations restored the growth fitness of reduced-genome Escherichia coli.

Journal of bioscience and bioengineering - 1 Jul 2013

Hirokawa Yasutaka, Kawano Hiroaki, Tanaka-Masuda Kimie, Nakamura Noriko, Nakagawa Akira, Ito Mikito, Mori Hideo, Oshima Taku, Ogasawara Naotake

Abstract excerpt

Microbes with smaller genomes would be better chassis for analysis, design, and improvement in the fields of metabolic engineering, synthetic biology, and molecular breeding. To create an Escherichia coli strain with a smaller genome, we used a stepwise genome reduction approach. Beginning with strain MGF-01, which has a genome of 3.62 megabase pairs (Mbp), we generated two E. coli K-12 strains without any...

Topics

DNA Repair
Down-Regulation
Escherichia coli
Escherichia coli Proteins
Gene Expression Profiling
Genome Size
Genome, Bacterial
Metabolic Engineering
Molecular Chaperones
Mutation

Source

Open in PubMed

Identifiers

PMID: 23477741
DOI: 10.1016/j.jbiosc.2013.01.010

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics