Article
Pulsed-field gel electrophoresis in the measurement of DNA double-strand break repair in xrs-6 and CHO cell lines: DNA degradation under some conditions interferes with the assessment of double-strand break rejoining.
Radiation research - 1 Jun 1992
Whitaker S J, McMillan T J
Abstract excerpt
Pulsed-field gel electrophoresis (PFGE) is a sensitive assay for DNA double-strand breaks (DSBs). The application of PFGE to the measurement of DSB rejoining requires attention to conditions after irradiation. When cells are irradiated and incubated while encapsulated in agarose, DNA DSB rejoinin...
Topics
- Animals
- CHO Cells
- Cell Line
- Cricetinae
- DNA
- DNA Damage
- DNA Repair
- Dose-Response Relationship, Radiation
- Electrophoresis, Gel, Pulsed-Field
- Mutation