Article
A method to monitor replication fork progression in mammalian cells: nucleotide excision repair enhances and homologous recombination delays elongation along damaged DNA.
Nucleic acids research - 10 Nov 2004
Johansson Fredrik, Lagerqvist Anne, Erixon Klaus, Jenssen Dag
Abstract excerpt
The capacity to rescue stalled replication forks (RFs) is important for the maintenance of cell viability and genome integrity. Here, we have developed a novel method for monitoring RF progression and the influence of DNA lesions on this process. The method is based on the principle that each RF is expected to be associated with a pair of single-stranded ends, which can be analyzed by employing strand separation...
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