Article
Pulsed field gel electrophoresis labeling method to study the pattern of Saccharomyces cerevisiae chromosomal DNA synthesis during the G1/S phase of the cell cycle.
Analytical biochemistry - 1 May 1995
Jong A Y, Wang B, Zhang S Q
Abstract excerpt
Yeast Saccharomyces cerevisiae is an excellent model to study eukaryotic DNA replication. Since yeast lacks thymidine kinase, it is difficult to assay DNA synthesis. In this report, a novel approach called the pulsed field gel electrophoresis (PFGE) labeling method is used to investigate yeast chromosomal DNA synthesis. In this method, yeast cells are first labeled by 32P in vivo and chromosomal DNA molecules are...
Topics
- Agglutinins
- Cell Cycle
- DNA Replication
- DNA, Fungal
- Electrophoresis, Gel, Pulsed-Field
- G1 Phase
- Genome, Fungal
- Hydroxyurea
- Kinetics
- Mating Factor
- Mutation