Article
A typing system for the major histocompatibility complex class I chain related genes A and B using polymerase chain reaction with sequence-specific primers.
Genetic testing - 1 Jan 2005
Rees M T, Downing J, Darke C
Abstract excerpt
The Major Histocompatibility Complex (MHC) class I chain related (MIC) A and B genes are important additional loci within the MHC. We have developed a MICA and MICB typing system using the polymerase chain reaction with sequence-specific primers (PCR-SSP), which operates under the same conditions as our routine HLA-A, -B, and -C typing method. We designed 95 primers in 84 SSP mixtures for MICA and 39 primers in...
Topics
- DNA Primers
- Genotype
- Histocompatibility Antigens Class I
- Humans
- Polymerase Chain Reaction
