Article
Human monocytes differentiate into macrophages under the influence of human KPB-M15 conditioned medium.
Journal of immunological methods - 1 Apr 2005
Gersuk Geoffrey, Hiraoka Atsunobu, Marr Kieren A
Abstract excerpt
Culture medium conditioned by the L929 murine fibroblast cell line contains macrophage colony-stimulating factor (M-CSF), providing an alternative to recombinant M-CSF for in vitro generation of murine macrophages. No such alternative has been described for in vitro studies requiring human macrophages. We tested the differentiation of human blood monocytes into mature macrophages by culturing in media conditioned...
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