Article
Generation of mature murine monocytes from heterogeneous bone marrow and description of their properties.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society - 1 Sept 2011
Francke Alexander, Herold Joerg, Weinert Soenke, Strasser Ruth H, Braun-Dullaeus Ruediger C
Abstract excerpt
Monocytes are involved in a wide range of physiological and pathological processes, many of which are studied in mouse models. Current protocols to isolate murine monocytes are few and result in unsatisfactory cell yield and purity. Here, we describe a novel approach to efficiently differentiate large numbers of mature inflammatory monocytes from heterogeneous bone marrow cell suspensions. Bone marrow cell...
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